Invertase reversibly immobilized onto polyethylenimine-grafted poly(GMA-MMA) beads for sucrose hydrolysis
Yükleniyor...
Tarih
2006
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The epoxy group containing poly(glycidyl methacrylate-co-methyl methacrylate) poly(GMA-MMA) beads were prepared by suspension polymerisation and the beads surface were grafted with polyethylenimine (PEI). The PEI-grafted beads were then used for invertase immobilization via adsorption. The immobilization of enzyme onto the poly(GMA-MMA)-PEI beads from aqueous solutions containing different amounts of invertase at different pH was investigated in a batch system. The maximum invertase immobilization capacity of the poly(GMA-MMA)-PEI beads was about 52 mg/g. It was shown that the relative activity of immobilized invertase was higher then that of the free enzyme over broader pH and temperature ranges. The Michaelis constant (K-m) and the maximum rate of reaction (V-max) were calculated from the Lineweaver-Burk plot. The K-m and V-max values of the immobilized invertase were larger than those of the free enzyme. The immobilized enzyme had a long-storage stability (only 6% activity decrease in 2 months) when the immobilized enzyme preparation was dried and stored at 4 degrees C while under wet condition 43% activity decrease was observed in the same period. After inactivation of enzyme, the poly(GMA-MMA)-PEI beads can be easily regenerated and reloaded with the enzyme for repeated use. (c) 2005 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
invertase, immobilized enzyme, affinity beads, adsorption, enzyme reactor
Kaynak
Journal Of Molecular Catalysis B-Enzymatic
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
38
Sayı
3-6
Künye
closedAccess
