Invertase reversibly immobilized onto polyethylenimine-grafted poly(GMA-MMA) beads for sucrose hydrolysis

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dc.contributor.authorArıca, M. Yakup
dc.contributor.authorBayramoğlu, Gülay
dc.date.accessioned2020-06-25T17:41:01Z
dc.date.available2020-06-25T17:41:01Z
dc.date.issued2006
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe epoxy group containing poly(glycidyl methacrylate-co-methyl methacrylate) poly(GMA-MMA) beads were prepared by suspension polymerisation and the beads surface were grafted with polyethylenimine (PEI). The PEI-grafted beads were then used for invertase immobilization via adsorption. The immobilization of enzyme onto the poly(GMA-MMA)-PEI beads from aqueous solutions containing different amounts of invertase at different pH was investigated in a batch system. The maximum invertase immobilization capacity of the poly(GMA-MMA)-PEI beads was about 52 mg/g. It was shown that the relative activity of immobilized invertase was higher then that of the free enzyme over broader pH and temperature ranges. The Michaelis constant (K-m) and the maximum rate of reaction (V-max) were calculated from the Lineweaver-Burk plot. The K-m and V-max values of the immobilized invertase were larger than those of the free enzyme. The immobilized enzyme had a long-storage stability (only 6% activity decrease in 2 months) when the immobilized enzyme preparation was dried and stored at 4 degrees C while under wet condition 43% activity decrease was observed in the same period. After inactivation of enzyme, the poly(GMA-MMA)-PEI beads can be easily regenerated and reloaded with the enzyme for repeated use. (c) 2005 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.molcatb.2005.12.006
dc.identifier.endpage138en_US
dc.identifier.issn1381-1177
dc.identifier.issue3-6en_US
dc.identifier.scopus2-s2.0-33644499137
dc.identifier.scopusqualityN/A
dc.identifier.startpage131en_US
dc.identifier.urihttps://doi.org/10.1016/j.molcatb.2005.12.006
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3608
dc.identifier.volume38en_US
dc.identifier.wosWOS:000236238800003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Molecular Catalysis B-Enzymatic
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectinvertaseen_US
dc.subjectimmobilized enzymeen_US
dc.subjectaffinity beadsen_US
dc.subjectadsorptionen_US
dc.subjectenzyme reactoren_US
dc.titleInvertase reversibly immobilized onto polyethylenimine-grafted poly(GMA-MMA) beads for sucrose hydrolysisen_US
dc.typeArticle

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