Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds

Basit Öğe Kaydı
dc.authoridErdemli, Ozge/0000-0001-8606-8863
dc.contributor.authorYelkuvan, Evrim Meric
dc.contributor.authorErdemli, Ozge
dc.contributor.authorYilmaz, Bengi
dc.contributor.authorAkturk, Omer
dc.date.accessioned2025-01-21T16:33:23Z
dc.date.available2025-01-21T16:33:23Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCollagen type I is the main structural unit in skin tissue and is therefore used preferentially in skin tissue scaffolds. However, collagen-based 3D scaffolds have weak aqueous stability and degradation profiles in their uncross-linked states and chemical cross -linking reagents arise toxicity concerns, which generally restrict the spectrum of their biomedical applicability. Here, the research goal is to photochemically cross-link collagen type I with rose bengal (RB) when subjected to green laser light and to investigate the effect of silk sericin-capped gold nanoparticles (S-AuNP) when incorporated into scaffolds on the cross-linking process and thus on the scaffold properties. All the collagen scaffolds, that is plain collagen (C), collagen/S-AuNP (C-Au), cross-linked collagen (C-RBL), and cross-linked collagen/S-AuNP (C-AuRBL) were characterized for their potential as skin tissue scaffolds. C-AuRBL group had the best thermal stability, resistance to enzymatic degradation, and more uniform pore size distribution. None of the groups had cytotoxicity (cell viability > 70%) regarding the microscopic observations and MTT cell viability assays for L929 fibroblasts. L929 fibroblasts and primary adult human epidermal keratinocytes (HEKa) were also separately seeded on C-AuRBL scaffolds and according to microscopy results, they could support the stimulation of adhesion, morphological changes, and spreading of both cells, thereby encouraging the usage of this fabrication strategy for prospective skin tissue scaffolds.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK), 2209-B Programme [1139B411702625]; Bioengineering Department of Kirikkale University; REMODERM Medical Ltd Company
dc.description.sponsorshipThis project was funded by the Scientific and Technological Research Council of Turkey (TUBITAK), 2209-B Programme (No: 1139B411702625) with the partnership of the Bioengineering Department of Kirikkale University and REMODERM Medical Ltd Company. All the synthesis and characterization tests were conducted in the Bioengineering Department of Kirikkale University and the Scientific and Technological Researches Application and Investigation Center of Kirikkale University (KUBTUAM) in Turkey. Special thanks to undergraduate students of the Bioengineering Department of Kirikkale University (Kubra Kayaalp, Saadet Elif Gul, Ayfer Karaaslan, Funda Kara, Busra Kicki, and Melike Tugce Uzun) for their valuable bits of help in the experiments. The Zetasizer, CTEM, SEM, Mercury Porosimeter, ATR-FTIR, TGA, and DMA analyses were conducted in the Central Laboratory of Middle East Technical University (METU) in Turkey (R & D Training and Measurement Center).
dc.identifier.doi10.55730/1300-0527.3521
dc.identifier.endpage115
dc.identifier.issn1300-0527
dc.identifier.issue1
dc.identifier.pmid37720862
dc.identifier.scopus2-s2.0-85151081110
dc.identifier.scopusqualityQ3
dc.identifier.startpage101
dc.identifier.trdizinid1158661
dc.identifier.urihttps://doi.org/10.55730/1300-0527.3521
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay1158661
dc.identifier.urihttps://hdl.handle.net/20.500.12587/23791
dc.identifier.volume47
dc.identifier.wosWOS:000986980500011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectCollagen type I; sericin; gold nanoparticles; green laser light; in vitro biocompatibility; skin tissue scaffolds
dc.titleEvaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu