Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives on the enzyme activity

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dc.contributor.authorTemel, Yusuf
dc.contributor.authorKoçyigit, Ümit M.
dc.contributor.authorTaysi, M. Şerif
dc.contributor.authorGökalp, Faik
dc.contributor.authorGürdere, Meliha Burcu
dc.contributor.authorBudak, Yakup
dc.contributor.authorÇiftci, Mehmet
dc.date.accessioned2020-06-25T18:29:41Z
dc.date.available2020-06-25T18:29:41Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionTemel, Yusuf/0000-0001-8148-3718; GULCIN, Ilhami/0000-0001-5993-1668; Ceylan, Mustafa/0000-0002-9184-4385; gokalp, faik/0000-0003-4363-3839
dc.description.abstractThe use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S-transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88-fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS-PAGE method and showed a single band. In addition, inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7methanoisoindole-1,3(2H)-dion derivatives (1a-g) were investigated on the enzyme activity. The inhibition parameters (IC50 and K-i values) were calculated for these compounds. IC50 values of these derivatives (1a-e) were found as 23.00, 15.75, 115.50, 10.00, and 28.75M, respectively. K-i values of these derivatives (1a-e) were calculated in the range of 3.04 +/- 0.50 to 131.50 +/- 32.50M. However, for f and g compounds, the inhibition effects on the enzyme were not found.en_US
dc.description.sponsorshipBingol UniversityBingol University [BAP-SSHMYO.2017.00.001]en_US
dc.description.sponsorshipBingol University, Grant/Award Number: BAP-SSHMYO.2017.00.001en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/jbt.22034
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue3en_US
dc.identifier.pmid29350485
dc.identifier.scopus2-s2.0-85040728747
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/jbt.22034
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7423
dc.identifier.volume32en_US
dc.identifier.wosWOS:000427566900004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Biochemical And Molecular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectQuail liveren_US
dc.subjectGSTen_US
dc.subjectinhibitionen_US
dc.subjectmethanoisoindoleen_US
dc.subjectenzyme purificationen_US
dc.titlePurification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives on the enzyme activityen_US
dc.typeArticle

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