The effect of G-CSF in an experimental MRSA graft infection in mice

Basit Öğe Kaydı
dc.contributor.authorAğalar, Canan
dc.contributor.authorEroğlu, Erol
dc.contributor.authorSari, Mustafa
dc.contributor.authorSari, Aliye
dc.contributor.authorDaphan, Çağatay
dc.contributor.authorAğalar, Fatih
dc.date.accessioned2020-06-25T17:40:26Z
dc.date.available2020-06-25T17:40:26Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.descriptionEurosurgery 2000 Meeting -- JUN 21-24, 2000 -- ISTANBUL, TURKEY
dc.description.abstractWound infection after prosthetic material implantation is a troublesome complication with an incidence of 2% to 10%. The effect of granulocyte colony-stimulating factor (G-CSF) was studied in an experimental methicillin-resistant Staphylococcus aureus (MRSA) graft infection model. Eighty adult mice were used. Under general anesthesia an abdominal incision of 2 cm in length was performed. A subcutaneous cavity of 2 x 2 cm in size was created. Polypropylene mesh pieces of 2 x 1 cm and MRSA solution of 0.1 ml of 10(8) CFU/mL were used. G-CSF was applied systemically or locally in a dosage of 0.02 MU/30 g body weight. There were 8 groups: group I, wound + MRSA; group II, wound + mesh + MRSA; group III, wound + mesh + MRSA + G-CSF (ip, 48 h before operation); group IV, wound + mesh + MRSA + G-CSF (ip, 24 h before operation); group V, wound + mesh + MRSA + G-CSF (locally, into the cavity); group VI, wound + mesh (incubated in G-CSF solution for 4 h) + MRSA; group VII, wound + mesh + MRSA + G-CSF, ip, 24 h from operation; and group VIII (positive control group), wound + mesh + MRSA + Teicoplanin (0.03 mg/30 g body weight, ip, 1/2 h before operation). Three days after, animals were killed and incisions were examined for possible infection or abscess formation and wound failure. Meshes were removed; after vortexing and dilution, samples were incubated with 5% agar media. Results of bacterial incubation were evaluated 24 h and 48 h later. There were symptoms of wound infection and abscess formation in all groups except group VIII. In group VIII, MRSA was isolated in 7 events with a colony count below 10(3). Bacterial counts were above 106 (10(6)-10(8)) in all other groups. Thus, it was observed that wound infection could be created with this model, but G-CSF could not prevent the development of wound infection, whether it was administered systemically or locally. Teicoplanin decreased the number of colony-forming units of MRSA, and prevents wound infection in this MRSA wound infection model.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1080/08941930500248581
dc.identifier.endpage231en_US
dc.identifier.issn0894-1939
dc.identifier.issn1521-0553
dc.identifier.issue5en_US
dc.identifier.pmid16249165
dc.identifier.scopus2-s2.0-32144438125
dc.identifier.scopusqualityQ1
dc.identifier.startpage227en_US
dc.identifier.urihttps://doi.org/10.1080/08941930500248581
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3439
dc.identifier.volume18en_US
dc.identifier.wosWOS:000233300500003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal Of Investigative Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectforeign-body infectionen_US
dc.subjectG-CSFen_US
dc.subjectMRSAen_US
dc.subjectTeicoplaninen_US
dc.titleThe effect of G-CSF in an experimental MRSA graft infection in miceen_US
dc.typeArticle

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