Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line

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dc.contributor.authorYirtici, Umit
dc.contributor.authorGoger, Fatih
dc.contributor.authorSarimahmut, Mehmet
dc.contributor.authorErgene, Aysun
dc.date.accessioned2020-06-25T18:23:39Z
dc.date.available2020-06-25T18:23:39Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.descriptionYIRTICI, Umit/0000-0002-0142-6105; Sarimahmut, Mehmet/0000-0003-2647-5875
dc.description.abstractThe main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that caused a cytotoxic effect was determined using MTT and ATP assays. The Centaurea fenzlii Reichardt dichloromethane extracts-ethyl acetate fractions (CFDCM-EAF) exhibited a stronger growth-inhibitory effect on MCF-7 cells (45.771 mu g/mL). The apoptotic effect was studied using double staining and flow cytometry. The death rate in the cells treated with the CFDCM-EAF IC50 dose was approximately 90%: 9.2% living cells, 22.8% necrotic cells, 62.3% late apoptotic cells, and 5.8% early apoptotic cells. Structural analysis of the CFDCM-EAF, which indicated significant cytotoxic effects, was performed using chromatographic methods. Hispidulin was the major component of the CFDCM-EAF by LC-APCI-MS/MS analysis.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110T905]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK Project No. 110T905). The authors would like to thank E Ulukaya (Prof in Faculty of Medicine at Uludag University) and N Kirimer (Prof in Faculty of Pharmacy at Anadolu University) for their comments on experiments, and F Yilmaz for her technical assistance.en_US
dc.identifier.citationYırtıcı Ü., Göger F., Sarımahmut M., Ergene A. (2017). Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line. Turkish Journal of Biology, 41(2), 370 - 377.en_US
dc.identifier.doi10.3906/biy-1609-74
dc.identifier.endpage377en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85018810082
dc.identifier.scopusqualityQ1
dc.identifier.startpage370en_US
dc.identifier.trdizinid244114
dc.identifier.urihttps://doi.org/10.3906/biy-1609-74
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7155
dc.identifier.volume41en_US
dc.identifier.wosWOS:000400179800013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEndemic Centaurea fenzlii Reichardten_US
dc.subjectMCF-7en_US
dc.subjectdichloromethane extract- ethyl acetate fractionen_US
dc.subjectcytotoxicityen_US
dc.subjectapoptotic effecten_US
dc.titleCytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell lineen_US
dc.typeArticle

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