eNOS and iNOS trigger apoptosis in the brains of sheep and goats naturally infected with the border disease virus

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Küçük Resim

Tarih

2015

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

F Hernandez

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

In this study, apoptotic and anti-apoptotic mechanisms and if present, which pathway to trigger the apoptosis in the brains of Border Disease Virus (BDV) infected lambs (n=10) and goat kids (n=5) were investigated. Briefly, apoptotic (caspase 3, caspase 9) and anti-apoptotic markers (Bcl-2), cytokine response (TNF-alpha, INF-gamma), reactive gliosis and myelin loss were examined. eNOS, iNOS, caspase 9, caspase 3 and GFAP expressions were higher in BDV infected tissues compared to control animals (6 kids and 6 lambs) (p<0.05). Double immunoperoxidase test revelaed that TUNEL positive apoptotic cells showed significant association with increased eNOS-iNOS and iNOS-BDV expressions. However, no significant differences were found for TNFR1, TNF-alpha and INF-gamma expressions in BD (p>0.05). There was a positive correlation between the intensity of myelin loss, GFAP activity and severity of infection. Inconclusion, as a novel finding, it is established that eNOS and iNOS overexpressions are co-associated with apoptosis in BDV infected neurons and neuroglia. The results also strongly suggested that BDV infected apoptotic cells mainly prefer the intrinsic pathway that might be most likely related to increased nitric oxide levels.

Açıklama

DINCEL, Gungor Cagdas/0000-0002-6985-3197

Anahtar Kelimeler

Apoptosis, Border disease, eNOS, iNOS

Kaynak

Histology And Histopathology

WoS Q Değeri

Q3

Scopus Q Değeri

Q2

Cilt

30

Sayı

10

Künye

closedAccess