The effect of different irrigation solutions and activation techniques on the expression of growth factors from dentine of extracted premolar teeth

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dc.contributor.authorHancerliogullari, Dilek
dc.contributor.authorErdemir, Ali
dc.contributor.authorKisa, Ucler
dc.date.accessioned2025-01-21T16:45:07Z
dc.date.available2025-01-21T16:45:07Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractAim To evaluate in a laboratory study, the effect of different canal irrigant solutions and activation techniques on transforming growth factor (TGF-beta 1), insulin growth factor-1 (IGF-1), bone morphogenetic protein-7 (BMP-7) and vascular endothelial growth factor-A (VEGF-A) release levels from the dentine of extracted premolar teeth. Methodology Seventy premolar teeth with single root and canal were used. The lengths of the root segments were standardized to 12 mm, and the root canals were prepared up to size 100 with hand files. All surfaces of the teeth were covered with nail polish except the inner root canal surface. The root canals were irrigated with 1.5% NaOCl. Ten teeth were allocated to the control group. The remaining sixty teeth were divided into 2 main groups according to the chelating agent used (17% EDTA, 10% Citric acid; CA) and 3 subgroups (n = 10) according to irrigation activation technique (conventional syringe irrigation (CSI), passive ultrasonic irrigation (PUI) and Er:YAG laser activation). After the activation procedure, the root segments were placed into eppendorf tubes containing 1 mL of phosphate-buffered saline solution and kept at 37celcius. TGF-beta 1, IGF-1, BMP-7 and VEGF-A release levels from dentine were measured using the enzyme-linked immunosorbent assay (ELISA) method at 24 h and at day 7. The volume of root canals was calculated using cone-beam computed tomography. The growth factor levels were calculated in ng/mL except VEGF-A (pg/ml). Normality analysis of the data was evaluated with the Kolmogorov-Smirnov test. Statistical analysis was performed using the Mann-Whitney-U and Wilcoxon tests. Results Regardless of the activation type and sampling time, EDTA caused significantly more IGF release than did CA, whereas EDTA and CA were equally effective for the release of the other growth factors. For either EDTA or CA, the lowest and highest growth factor release levels were observed in the CSI and Er:YAG laser groups, respectively (p < .05). All of the growth factors were released significantly more at 24 h than on day 7 (p < .05). Conclusions Irrigation activation techniques with EDTA or CA increased the release levels of all growth factors from the dentine of canal walls in extracted premolar teeth.
dc.description.sponsorshipScientific Research Projects Coordination Center of Kirikkale University, Kirikkale/Turkey [2019/088]
dc.description.sponsorshipThis research, the thesis of Dt. Dilek Hancerliogullari, was supported by Scientific Research Projects Coordination Center (Project number: 2019/088) of Kirikkale University, Kirikkale/Turkey.
dc.identifier.doi10.1111/iej.13589
dc.identifier.endpage1924
dc.identifier.issn0143-2885
dc.identifier.issn1365-2591
dc.identifier.issue10
dc.identifier.pmid34115394
dc.identifier.scopus2-s2.0-85109600592
dc.identifier.scopusqualityQ1
dc.identifier.startpage1915
dc.identifier.urihttps://doi.org/10.1111/iej.13589
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25591
dc.identifier.volume54
dc.identifier.wosWOS:000672030300001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofInternational Endodontic Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectcitric acid; EDTA; Er; YAG laser; growth factors; regenerative endodontics; ultrasonic
dc.titleThe effect of different irrigation solutions and activation techniques on the expression of growth factors from dentine of extracted premolar teeth
dc.typeArticle

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