The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity

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dc.contributor.authorSozmen, Mahmut
dc.contributor.authorDevrim, Alparslan K.
dc.contributor.authorKabak, Yonca B.
dc.contributor.authorDevrim, Tuba
dc.contributor.authorSudagidan, Mert
dc.date.accessioned2020-06-25T18:29:41Z
dc.date.available2020-06-25T18:29:41Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionSozmen, Mahmut/0000-0001-7976-4051
dc.description.abstractPeriostin is an extracellular matrix protein from fasciclin family, and it plays an important role in the cell adhesion, migration, and growth of the organism. Periostin prevents apoptosis while stimulating cardiomyocytes. The present study was designed to investigate cardioprotective effects of the recombinant murine periostin peptide administration in a rat model of isoproterenol (ISO)-induced myocardial injury. The experiment was performed on 84 adult male Sprague Dawley rats in 4 groups (n = 21): control group (1), periostin-treated group (2), ISO-treated group (3), and ISO + periostin-treated group (4). The groups were further divided into three subgroups based on the duration of the experiment in which rats were killed on days 1, 7, and 28 (n = 7). Growth factors (VEGF, ANGPT, FGF-2, TGF beta), mitosis and apoptosis (Bcl-2, Bax, PCNA, Ki-67, phospho-histone H3), cell cycle activators and inhibitors (cyclin D1, D2, A2, Cdc2), the origin of regenerating cells (cKit and CD45) mRNA were detected using quantitative real-time polymerase chain reaction (PCR) and PCR array. Immunohistochemistry staining was used to directly detect protein level and distribution in the heart tissues. Administration of periostin following ISO-induced cardiotoxicity revealed that periostin alleviated deleterious effects of ISO through several pathways: (1) periostin induced mitotic activity of endothelial/fibroblastic cells, (2) periostin drives cardiomyocytes into S and M phases, thus promoting proliferation of cardiomyocytes, (3) periostin contributed to collagen degradation, tissue remodeling, and reduced cardiac fibrosis during the healing process following myocardial damage while preserving tissue matrix, (4) periostin stimulated angiogenesis by upregulating THBS1, TGFB2, and HGF genes, (5) periostin regulated cell growth and proliferation while maintaining cell shape and cellular muscle contractions (ACTB) and functioned as chemoattractant factor (CCL2) at the beginning of myocardial damage.en_US
dc.description.sponsorshipTurkish Scientific Research Council (TUBITAK-TOVAG), Ankara, Turkey [114O734]en_US
dc.description.sponsorshipThis project was financially supported by Turkish Scientific Research Council (TUBITAK-TOVAG; Project No: 114O734), Ankara, Turkey.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s12012-017-9426-y
dc.identifier.endpage160en_US
dc.identifier.issn1530-7905
dc.identifier.issn1559-0259
dc.identifier.issue2en_US
dc.identifier.pmid28895052
dc.identifier.scopus2-s2.0-85029029042
dc.identifier.scopusqualityQ1
dc.identifier.startpage142en_US
dc.identifier.urihttps://doi.org/10.1007/s12012-017-9426-y
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7420
dc.identifier.volume18en_US
dc.identifier.wosWOS:000426954900004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Incen_US
dc.relation.ispartofCardiovascular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCardiotoxicityen_US
dc.subjectIsoproterenolen_US
dc.subjectPeriostinen_US
dc.subjectRaten_US
dc.titleThe Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicityen_US
dc.typeArticle

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