Autologous Blood Pleurodesis in Rats to Elucidate the Amounts of Blood Required for Reliable and Reproducible Results

Basit Öğe Kaydı
dc.contributor.authorÖzpolat, Berkant
dc.contributor.authorGazyağcı, Serkal
dc.contributor.authorGözübüyük, Alper
dc.contributor.authorAyva, Şebnem
dc.contributor.authorAtinkaya, Cansel
dc.date.accessioned2020-06-25T17:49:27Z
dc.date.available2020-06-25T17:49:27Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.descriptionOzpolat, Berkant/0000-0002-6203-7306; GAZYAGCI, SERKAL/0000-0002-0043-6942
dc.description.abstractBackground. Pleurodesis is used in the treatment of spontaneous pneumothorax or refractory pleural effusions of different etiologies. Several agents have been employed, but many questions remain unanswered about their effectiveness and toxicity. Use of autologous blood pleurodesis in clinical practice has been described in the literature without any clear consensus regarding its efficacy. Experimental studies using this technique are limited to a single study in rabbits. We performed a prospective, randomized, observer-blinded, controlled study to evaluate the safety and efficacy of increasing doses of autologous blood pleurodesis in a novel rat model. Materials and Methods. Twenty-eight albino Wistar rats were divided into four groups. Groups 1, 2, and 3 were the study groups and group 4 was the control group, with seven animals in each group. Groups 1, 2, and 3 were given autologous blood, 1mL/kg, 2mL/kg, 3mL/kg, respectively, and group 4 (control) was given only 2mL/kg saline intrapleurally. The rats were sacrificed on postoperative day 30. The surfaces were graded by macroscopic (visible adhesion formation) and microscopic (inflammation and fibrosis) examination. Results. Macroscopically, group 2 and group 3 developed significantly more adhesions; 3mL/kg autologous blood produced the most significant pleurodesis with generalized adhesions seen between visceral, parietal, and mediastinal pleura. Microscopic examination showed that all study groups developed an inflammatory response at the site of blood injection. There were no pathologic changes in ipsilateral and contralateral lung parenchyma. Conclusions. Autologous blood at doses 2-3mL/kg were shown to be effective to produce adhesions in 30 d, and the results were highly reproducible in all rats. We propose that the occasional negative results obtained in humans may be related to an insufficient amount of injected blood, as observed in our rat model. (C) 2010 Elsevier Inc. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jss.2009.01.027
dc.identifier.endpage232en_US
dc.identifier.issn0022-4804
dc.identifier.issue2en_US
dc.identifier.pmid19524261
dc.identifier.scopus2-s2.0-77951954389
dc.identifier.scopusqualityQ1
dc.identifier.startpage228en_US
dc.identifier.urihttps://doi.org/10.1016/j.jss.2009.01.027
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4720
dc.identifier.volume161en_US
dc.identifier.wosWOS:000277885300009
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofJournal Of Surgical Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectblooden_US
dc.subjectpleuraen_US
dc.subjectpleurodesisen_US
dc.subjectpneumothoraxen_US
dc.titleAutologous Blood Pleurodesis in Rats to Elucidate the Amounts of Blood Required for Reliable and Reproducible Resultsen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Autologous Blood Pleurodesis in Rats to Elucidate the Amounts of Blood Required for Reliable and Reproducible Results.pdf
Boyut:
700.01 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu