Effect of cyclodextrin-encapsulated β-carotene on progesterone production by bovine luteal cells
Yükleniyor...
Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Experiments were conducted to examine the effect of cyclodextrin-encapsulated beta -carotene on basal or cholesterol (cyclodextrin-encapsulated), LH and dibutyryl cyclic AMP (dbcAMP)stimulated progesterone production by bovine corpus luteum cells isolated from mid-luteal heifer ovaries by collagenase digestion. Cells were cultured with serum-free DMEM/Ham's F12 medium in serum pre-treated plastic culture dishes for periods of up to 11 days. Medium was replaced after 24h and thereafter every 48h. beta -carotene was added to cultures in a carrier molecule, dimethyl-beta -cyclodextrin, to facilitate dissolution. All treatments were started on day 3 of culture. Treatment of cells with 1 or 2 mu mol/l beta -carotene resulted in sharp inhibition of progesterone production. On the contrary, treatment of cells with 0.1 mu mol/l beta -carotene resulted in significant stimulation (P < 0.05) of both basal and cholesterol-stimulated progesterone secretion. The effect of <beta>-carotene on LH or dbcAMP-stimulated progesterone production was also examined. Treatment of cells with LH or dbcAMP always resulted in stimulation of progesterone secretion (P < 0.001). However, cells treated with LH plus <beta>-carotene or dbcAMP plus beta -carotene both produced significantly (P < 0.01) less progesterone relative to those cells treated with LH or dbcAMP alone on days 7, 9 and 11 of culture. These results indicate that <beta>-carotene can enhance luteal steroidogenesis when present at low concentrations but is inhibitory at higher concentrations and that encapsulation of beta -carotene in cyclodextrin is an effective method of supplying it to cells in culture. (C) 2000 Elsevier Science B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
beta-carotene, cattle-endocrinology, luteal cells, cyclodextrin, progesterone
Kaynak
Animal Reproduction Science
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
64
Sayı
3-4
Künye
closedAccess
