Effect of cyclodextrin-encapsulated β-carotene on progesterone production by bovine luteal cells

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dc.contributor.authorArikan, S
dc.contributor.authorRodway, RG
dc.date.accessioned2020-06-25T17:34:40Z
dc.date.available2020-06-25T17:34:40Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractExperiments were conducted to examine the effect of cyclodextrin-encapsulated beta -carotene on basal or cholesterol (cyclodextrin-encapsulated), LH and dibutyryl cyclic AMP (dbcAMP)stimulated progesterone production by bovine corpus luteum cells isolated from mid-luteal heifer ovaries by collagenase digestion. Cells were cultured with serum-free DMEM/Ham's F12 medium in serum pre-treated plastic culture dishes for periods of up to 11 days. Medium was replaced after 24h and thereafter every 48h. beta -carotene was added to cultures in a carrier molecule, dimethyl-beta -cyclodextrin, to facilitate dissolution. All treatments were started on day 3 of culture. Treatment of cells with 1 or 2 mu mol/l beta -carotene resulted in sharp inhibition of progesterone production. On the contrary, treatment of cells with 0.1 mu mol/l beta -carotene resulted in significant stimulation (P < 0.05) of both basal and cholesterol-stimulated progesterone secretion. The effect of <beta>-carotene on LH or dbcAMP-stimulated progesterone production was also examined. Treatment of cells with LH or dbcAMP always resulted in stimulation of progesterone secretion (P < 0.001). However, cells treated with LH plus <beta>-carotene or dbcAMP plus beta -carotene both produced significantly (P < 0.01) less progesterone relative to those cells treated with LH or dbcAMP alone on days 7, 9 and 11 of culture. These results indicate that <beta>-carotene can enhance luteal steroidogenesis when present at low concentrations but is inhibitory at higher concentrations and that encapsulation of beta -carotene in cyclodextrin is an effective method of supplying it to cells in culture. (C) 2000 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0378-4320(00)00202-5
dc.identifier.endpage160en_US
dc.identifier.issn0378-4320
dc.identifier.issue3-4en_US
dc.identifier.pmid11121892
dc.identifier.scopus2-s2.0-0034731247
dc.identifier.scopusqualityQ1
dc.identifier.startpage149en_US
dc.identifier.urihttps://doi.org/10.1016/S0378-4320(00)00202-5
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2832
dc.identifier.volume64en_US
dc.identifier.wosWOS:000166226300002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeta-caroteneen_US
dc.subjectcattle-endocrinologyen_US
dc.subjectluteal cellsen_US
dc.subjectcyclodextrinen_US
dc.subjectprogesteroneen_US
dc.titleEffect of cyclodextrin-encapsulated β-carotene on progesterone production by bovine luteal cellsen_US
dc.typeArticle

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