Effect of nitrogen mustard, a vesicant agent, on lymphocyte energy metabolism
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Tarih
2006
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Walter De Gruyter Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: The vesicant agents sulfur and nitrogen mustards, which contain chloroethyl groups, are potent inhibitors of DNA synthesis and cell growth, likely changing the utilization of anaerobic glycolysis for energy generation. Methods: To investigate the effect of nitrogen mustard on cellular energy metabolism, lymphocytes treated with increasing doses of mechlorethamine (HN2), a nitrogen mustard and an analogue of sulfur mustard, were incubated with radiolabeled glucose. The rates of aerobic and anaerobic glycolysis were then determined. Results: Glycogen consumption was significantly higher in cells treated with HN2 in a dose-dependent manner compared to untreated cells. Similarly, the amount of end-product lactate was increased, but CO2 was reduced in HN2-treated cells. Conclusions: Lymphocytes normally use aerobic glycolysis under aerobic conditions, but energy metabolism predominantly involved anaerobic glycolysis after severe intoxication with mustard agent.
Açıklama
SERDAR, Muhittin A./0000-0002-3014-748X
Anahtar Kelimeler
energy metabolism, glycogen degradation, glycolysis, mechlorethamine, mustard
Kaynak
Clinical Chemistry And Laboratory Medicine
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
44
Sayı
10
Künye
closedAccess
