Effects of mancozeb, metalaxyl and tebuconazole on steroid production by bovine luteal cells in vitro

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dc.contributor.authorAtmaca, Nurgul
dc.contributor.authorArikan, Sevket
dc.contributor.authorEssiz, Dinc
dc.contributor.authorKalender, Hakan
dc.contributor.authorSimsek, Ozkan
dc.contributor.authorBilmen, Fatih Sultan
dc.contributor.authorKabakci, Ruhi
dc.date.accessioned2020-06-25T18:29:38Z
dc.date.available2020-06-25T18:29:38Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionKABAKCI, Ruhi/0000-0001-9131-0933
dc.description.abstractMancozeb, metalaxyl and tebucanazole are widely used pesticides in agriculture and industry to treat plant pathogenic fungi. Livestock may be exposed to such substances by consuming contaminated plants. The present study was designed to evaluate the effects of these three fungicides on bovine luteal cell steroidogenesis. Luteal slices from mid-cycle corpus luteum were dissociated into single cell suspension in aerated (02) culture media (DMEM/F12) by enzymatic digestion. The cells were incubated in newborn calf serum (10%) for 18 h and then with serum-free media containing mancozeb (0.01 mu M, 0.1 mu M, 1 mu M), tebuconazole (1 mu M, 10 mu M, 100 mu M) or metalaxyl (100 mu M, 500 mu M, 2500 mu M) for additional 96 h. The medium was replaced on day 1 and 3; and the retrieved medium was stored at - 20 degrees C until progesterone assay. Treatment of cells with three different fungicides induced dose dependent variable decrease in steroid synthesis during incubation periods. Incubation of cells with 1 pM mancozeb exhibited a 33% decline in steroid synthesis on day 3 and 48% decline on day 5 compared with controls. Treatment of cells with 100 M tebuconazole and 500 mu M metalaxyl resulted in a 65% and 31% decrease, respectively, in progesterone accumulation on day 5 of incubation. Fungicide induced suppressive effects on luteal steroidogenesis were as metalaxyl < tebuconazole < mancozeb. Results of the present study suggest that designated concentrations of all three fungicides studied might have varying degrees of adverse effects on luteal steroidogenesis.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-213O174]en_US
dc.description.sponsorshipThe present study was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK). Project No: TOVAG-213O174.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.etap.2018.03.009
dc.identifier.endpage118en_US
dc.identifier.issn1382-6689
dc.identifier.issn1872-7077
dc.identifier.pmid29558666
dc.identifier.scopus2-s2.0-85044005052
dc.identifier.scopusqualityQ1
dc.identifier.startpage114en_US
dc.identifier.urihttps://doi.org/10.1016/j.etap.2018.03.009
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7407
dc.identifier.volume59en_US
dc.identifier.wosWOS:000432507800015
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEnvironmental Toxicology And Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLuteal cellsen_US
dc.subjectFungicideen_US
dc.subjectMancozeben_US
dc.subjectMetalaxylen_US
dc.subjectTebuconazoleen_US
dc.subjectProgesteroneen_US
dc.titleEffects of mancozeb, metalaxyl and tebuconazole on steroid production by bovine luteal cells in vitroen_US
dc.typeArticle

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