Radioprotective effect of lycopene on chromosomal aberrations (CAs) induced by gamma radiation in human lymphocytes
Yükleniyor...
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Triveni Enterprises
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present study was carried out to evaluate the radioprotective role of lycopene on chromosomal aberrations (CAs) induced by gamma (gamma) radiation in human lymphocytes. For this purpose, we used in vitro dose-effect relationship, and correlate these data with statistical parameters. CAs were evaluated in terms of chromosome break, dicentric, tricentric, acentric, fragment and ring. Mitotic index (MI) and aberrant metaphase number (AMN) were also calculated. Nine groups of lymphocyte culture were prepared: (I) Control group did not received radiation or lycopene, (II) positive control; lymphocytes were treated only with 0.020 mu M lycopene, (III) radiation group; lymphocytes were treated with only 10 Gy gamma-radiation, (IV) lymphocytes were treated with 0.001, mu M lycopene before irradiation, (V) lymphocytes were treated with 0.005 mu M lycopene before irradiation, (VI) lymphocytes were treated with 0.010 mu M lycopene before irradiation, (VII) lymphocytes were treated with 0.015 mu M lycopene before irradiation, (VIII) lymphocytes were treated with 0.018 mu M lycopene before irradiation, (IX) lymphocytes were treated with treated with 0.020 mu M lycopene before irradiation. The results indicated that all lycopene-supplemented lymphocytes showed a lower CAs frequency than lymphocytes in only radiation treated group. It was seen that lycopene had a protective effect on CAs particularly at 0.010, 0.015 and 0.018 mu M concentrations, but this effect saturated at 0.020 mu M concentration. Besides, MI increased and AMN decreased depending on applied lycopene doses. In vitro results showed that the lycopene supplementation decreases frequency of CAs and its protective role against chromosome damages induced by radiation is dose-dependent until a certain stage.
Açıklama
Anahtar Kelimeler
Aberrant metaphase number, Chromosomal aberrations, Gamma radiation, Lycopene, Mitotic index
Kaynak
Journal Of Environmental Biology
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
30
Sayı
1
Künye
closedAccess
