Influence of scaffold composition over invitro osteogenic differentiation of hBMSCs and invivo inflammatory response
Yükleniyor...
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sage Publications Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
To understand the role of chitosan in chitosan-poly(butylene succinate) scaffolds (50% wt), 50%, 25%, and 0% of chitosan were used to produce different scaffolds. These scaffolds were invitro seeded and cultured with human bone marrow stromal cells in osteogenic conditions, revealing that higher percentage of chitosan showed enhanced cell viability over time, adhesion, proliferation, and osteogenic differentiation. Scaffolds were also implanted in cranial defects and iliac submuscular region in Wistar rats, and the results evidenced that chitosan-containing scaffolds displayed mild inflammatory response and good integration with surrounding tissues, showed by connective tissue colonization and the presence of new blood vessels. Scaffolds without chitosan-evidenced necrotic tissue in scaffolds' interior, proving that chitosan exerts a positive effect over cell behavior and displays a milder host inflammatory response invivo.
Açıklama
Bayram, Cem/0000-0001-8717-4668; Faria, Susana/0000-0001-8014-9902; Correlo, Vitor M/0000-0002-5516-7583; Neves, Nuno M/0000-0003-3041-0687; Reis, Rui L. L/0000-0002-4295-6129; Sol, Paula/0000-0003-0512-0928; Costa-Pinto, Ana Rita/0000-0002-4764-8539; UMinho, 3B's Research Group/0000-0002-5195-3456; Tuzlakoglu, Kadriye/0000-0002-7564-9213
Anahtar Kelimeler
biomaterials, tissue engineering, regenerative medicine, Chitosan, scaffolds
Kaynak
Journal Of Biomaterials Applications
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
28
Sayı
9
Künye
closedAccess
