In vitro antiproliferative/cytotoxic activity of 2,3 '-biindole against various cancer cell lines
Yükleniyor...
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technical Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole (2) may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action.
Açıklama
Erenler, Ramazan/0000-0002-0505-3190; Okten, Salih/0000-0001-9656-1803; Kul Koprulu, Tugba/0000-0001-9451-5715
Anahtar Kelimeler
2,3 '-Biindole, anticancer, cytotoxicity, apoptosis, antitopoisomerase, C6 cell line, HeLa cell line, HT29 cell line
Kaynak
Turkish Journal Of Biology
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
39
Sayı
1
Künye
Ökten, S., Erenler, R., Köprülü, T. K., Tekin, Ş. (2015). In vitro antiproliferative/cytotoxic activity of 2,3\'-biindole against various cancer cell lines. Turkish Journal of Biology, 39(1), 15 - 22.
