In vitro antiproliferative/cytotoxic activity of 2,3 '-biindole against various cancer cell lines

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dc.contributor.authorOkten, Salih
dc.contributor.authorErenler, Ramazan
dc.contributor.authorKoprulu, Tugba Kul
dc.contributor.authorTekin, Saban
dc.date.accessioned2020-06-25T18:15:46Z
dc.date.available2020-06-25T18:15:46Z
dc.date.issued2015
dc.departmentKırıkkale Üniversitesi
dc.descriptionErenler, Ramazan/0000-0002-0505-3190; Okten, Salih/0000-0001-9656-1803; Kul Koprulu, Tugba/0000-0001-9451-5715
dc.description.abstract2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole (2) may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action.en_US
dc.description.sponsorshipKirikkale University Research FundKirikkale University [2012/122]en_US
dc.description.sponsorshipThis study was financially supported by a grant from the Kirikkale University Research Fund (2012/122).en_US
dc.identifier.citationÖkten, S., Erenler, R., Köprülü, T. K., Tekin, Ş. (2015). In vitro antiproliferative/cytotoxic activity of 2,3\'-biindole against various cancer cell lines. Turkish Journal of Biology, 39(1), 15 - 22.en_US
dc.identifier.doi10.3906/biy-1402-60
dc.identifier.endpage22en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84919663332
dc.identifier.scopusqualityQ1
dc.identifier.startpage15en_US
dc.identifier.trdizinid214416
dc.identifier.urihttps://doi.org/10.3906/biy-1402-60
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6266
dc.identifier.volume39en_US
dc.identifier.wosWOS:000346847200003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject2,3 '-Biindoleen_US
dc.subjectanticanceren_US
dc.subjectcytotoxicityen_US
dc.subjectapoptosisen_US
dc.subjectantitopoisomeraseen_US
dc.subjectC6 cell lineen_US
dc.subjectHeLa cell lineen_US
dc.subjectHT29 cell lineen_US
dc.titleIn vitro antiproliferative/cytotoxic activity of 2,3 '-biindole against various cancer cell linesen_US
dc.typeArticle

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