What is the protective effect of metformin on rat ovary against ischemia-reperfusion injury?
Yükleniyor...
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
AimThe aim of this study was to investigate the protective effect of metformin on the rat ovary against ischemia-reperfusion injury. MethodsThirty-seven female Wistar albino rats were used in the study. The rats were divided into five groups, as follows: sham operation group (group 1); torsion group (group 2); torsion/detorsion+saline group (group 3); torsion/detorsion+low-dose metformin group (group 4); and torsion/detorsion+high-dose metformin group (group 5). The right ovary from each rat was evaluated histologically using hematoxylin-eosin staining, and the left ovaries were evaluated for tissue levels of the reduced-glutathione-to-oxidized-glutathione ratio, malondialdehyde (MDA), and caspase-3 activation. ResultsThe highest damage score was observed in group 3, and the lowest score was observed in group 1. The tissue caspase-3 activity levels of groups 2, 3, and 4 were significantly higher than those of group 1. The difference between group 1 and group 5 in terms of tissue caspase-3 activity was not significant (P=0.4). The reduced-glutathione-to-oxidized-glutathione ratio of group 1 was significantly higher than the ratios found in groups 2, 3, and 4. The tissue MDA level of group 1 was significantly lower than the levels found in groups 2, 3, 4, and 5. The tissue MDA level of group 5 was significantly lower than the levels in groups 3 and 4. ConclusionFrom both histopathological and biochemical analyses, the results of the study demonstrated that metformin has beneficial effects when it comes to attenuating ovarian ischemia-reperfusion injury.
Açıklama
Ozkan, Zehra Sema/0000-0001-9185-3663
Anahtar Kelimeler
ischemia-reperfusion injury, metformin, ovary, rat, reactive oxygen species
Kaynak
Journal Of Obstetrics And Gynaecology Research
WoS Q DeÄŸeri
Q4
Scopus Q DeÄŸeri
Q2
Cilt
44
Sayı
2
Künye
closedAccess
