Immunolocalization of 3β-hydroxysteroid dehydrogenase in normal and hyperplastic ram prostates
Yükleniyor...
Tarih
2006
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The enzyme 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) is essential in the synthesis of all steroids by cleaving dehydroepiandrosterone to androstenedione. In the present study, 3 beta-HSD immunoreactivity was investigated in the prostate of Akkaraman breed rams aged older than 3 years. Five normal and five hyperplastic ram prostates were processed for immunohistochernistry. Prostate hyperplasia was determined by histopathological evaluation of 375 ram prostate and confirmed with significantly (P < 0.01) increased number of cells expressing proliferating cell nuclear antigen (PCNA) immunoreactivity in the glandular epithelia. The 3 beta-HSD immunoreactivity with a variable intensity and pattern of distribution was present in the glandular epithelia and endothelia of blood vessels in normal and hyperplastic ram prostates. While immunoreactivity was focally present in some glands, some sections had a homogenous distribution. The presence of 3 beta-HSD immunoreactivity indicates that steroids are locally synthesized in the ram prostate. No differences in the distribution pattern of 3 beta-HSD immunoreactivity and the percentage of immunoreactive cells were observed between normal and hyperplastic prostates (P > 0.05), suggesting that locally produced steroids have little or no effect onthe pathogenesis of the ram prostate hyperplasia which affects a very small proportion of the ram population (5 out of 375). (c) 2005 Elsevier Inc. All rights reserved.
Açıklama
/0000-0002-0636-4214; ATMACA, HASAN TARIK/0000-0001-8379-4114
Anahtar Kelimeler
3 beta-hydroxysteroid dehydrogenase, hyperplasia, immunohistochemistry, ram, prostate
Kaynak
Domestic Animal Endocrinology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
30
Sayı
1
Künye
closedAccess
