The effect of colchicine and low-dose methotrexate on intestinal ischemia/reperfusion injury in an experimental model

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dc.contributor.authorBoybeyi, Ozlem
dc.contributor.authorGunal, Yasemin Dere
dc.contributor.authorAtasoy, Pinar
dc.contributor.authorKisa, Ucler
dc.contributor.authorAslan, Mustafa Kemal
dc.date.accessioned2020-06-25T18:12:06Z
dc.date.available2020-06-25T18:12:06Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.descriptionKISA, Ucler/0000-0002-8131-6810
dc.description.abstractAim: Intestinal ischemia/reperfusion (I/R) injury is a serious clinical condition. Colchicine and low-dose methotrexate have anti-inflammatory features. An experimental model was conducted to investigate the effect of colchicine and methotrexate on intestinal I/R injury. Methods: Twenty-four rats were included. Only laparotomy was done in control group (CG, n = 6). In experimental groups, superior mesenteric artery was occluded. After 1 h ischemia, reperfusion (1 h) was started by de-occlusion. 30 min before reperfusion, saline in sham group (SG,n: 6), colchicine (1 mg/kg) in colchicine group (CNG, n: 6), andmethotrexate (0.1 mg/kg) in methotrexate group (MTXG, n: 6) were infused intraperitoneally. Small intestines were harvested for evaluation of intestinal mucosal injury (Chiu score) and oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD). Results: Biochemically, MDA levels were significantly low in CG compared to SG, CNG, and MTXG (p < 0.05). NO levels were significantly low and SOD levels were significantly high in CG compared to MTXG (p < 0.05). Histopathologically, Chiu score was significantly low in CG compared to SG, CNG, and MTXG (p < 0.05), and significantly high in MTXG compared to SG and CNG (p b 0.05). Conclusion: The present experimental model caused I/R injury in rat intestines. Contrary to literature, it was found that methotrexate worsens and colchicine does not attenuate intestinal I/R injury. (C) 2014 Elsevier Inc. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jpedsurg.2014.01.057
dc.identifier.endpage1474en_US
dc.identifier.issn0022-3468
dc.identifier.issn1531-5037
dc.identifier.issue10en_US
dc.identifier.pmid25280648
dc.identifier.scopus2-s2.0-84908146270
dc.identifier.scopusqualityQ1
dc.identifier.startpage1471en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpedsurg.2014.01.057
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5732
dc.identifier.volume49en_US
dc.identifier.wosWOS:000343140200006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherW B Saunders Co-Elsevier Incen_US
dc.relation.ispartofJournal Of Pediatric Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIntestinal ischemiaen_US
dc.subjectIschemia/reperfusion injuryen_US
dc.subjectColchicineen_US
dc.subjectMethotrexateen_US
dc.subjectExperimentalen_US
dc.titleThe effect of colchicine and low-dose methotrexate on intestinal ischemia/reperfusion injury in an experimental modelen_US
dc.typeArticle

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