Effects of Thalidomide and rhGM-CSF on Carbohydrate Metabolism in HL-60 Acute Promyelocytic Leukemia Cell Line

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dc.contributor.authorBoyunaga, Hakan
dc.contributor.authorDikmen, Gunnur
dc.contributor.authorUral, A. Ugur
dc.contributor.authorMelekoglu, Abdullah
dc.date.accessioned2020-06-25T17:52:06Z
dc.date.available2020-06-25T17:52:06Z
dc.date.issued2011
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe purpose of this study is to investigate the in vitro effect of thalidomide and rhGM-CSF on energy metabolism rate in HL-60 cell lines. HL-60 cells were incubated with radiolabelled glucose for 4 hours. Following incubation, radiolabelled CO2 was isolated and collected in specially designed scintillation vials. The other end-products of carbohydrate catabolism collected via anion-exchange chromatography were analyzed using liquid scintillation. Protein and glycogen levels for each group were also determined. The study results indicated that the use of drugs for 2-days had no significant effect when compared with aerobic and anaerobic controls. However, groups treated with the drugs for 5 days showed significant differences. It was obvious that rhGM-CSF caused HL-60 leukemia cells to use aerobic glycolytic pathway for production of energy. When thalidomide and rhGM-CSF combination therapy results were compared with untreated control cells, it was observed that glycogen consumption was decreased by 50.37%, and CO2 production was increased by 94.03%. When compared with those of anaerobic controls, glycogen consumption and CO2 production rates were found to be decreased by 54.01% and increased by 96.59%, respectively. As a consequence, we found that those cell lines treated with combined use of Thalidomide and rhGM-CSF caused HL-60 leukemia cells to predominantly prefer aerobic glycolytic pathways for energy production.en_US
dc.identifier.citationBoyunağa, H., Dikmen, G., Ural, A. U., Melekoğlu, A. (2011). Effects of thalidomide and rhGM-CSF on carbohydrate metabolism in HL-60 Acute promyelocytic leukemia cell line. Uluslararası Hematoloji-Onkoloji Dergisi, 21(3), 170 - 175.en_US
dc.identifier.doi10.4999/uhod.11007
dc.identifier.endpage175en_US
dc.identifier.issn1306-133X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-80053642399
dc.identifier.scopusqualityQ4
dc.identifier.startpage170en_US
dc.identifier.trdizinid124033
dc.identifier.urihttps://doi.org/10.4999/uhod.11007
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5080
dc.identifier.volume21en_US
dc.identifier.wosWOS:000295573200007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherAkad Doktorlar Yayinevien_US
dc.relation.ispartofUhod-Uluslararasi Hematoloji-Onkoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectThalidomideen_US
dc.subjectMetabolismen_US
dc.subjectrhGM-CSFen_US
dc.subjectHL-60 cell lineen_US
dc.titleEffects of Thalidomide and rhGM-CSF on Carbohydrate Metabolism in HL-60 Acute Promyelocytic Leukemia Cell Lineen_US
dc.typeArticle

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