Oxidative DNA damage and total antioxidant status in rats during experimental gram-negative sepsis

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dc.contributor.authorKaymak, C.
dc.contributor.authorKadioglu, E.
dc.contributor.authorOzcagli, E.
dc.contributor.authorOsmanoglu, G.
dc.contributor.authorIzdes, S.
dc.contributor.authorAgalar, C.
dc.contributor.authorSardas, S.
dc.date.accessioned2020-06-25T17:44:34Z
dc.date.available2020-06-25T17:44:34Z
dc.date.issued2008
dc.departmentKırıkkale Üniversitesi
dc.descriptionSardas, Semra/0000-0001-5456-8636
dc.description.abstractSepsis and septic shock remains as leading cause of death in adult intensive care units. It is widely accepted that gram-negative bacteria and their endotoxins cause sepsis and septic shock, predominantly. Enhanced generation of reactive oxygen species may be responsible for tissue injury in septic shock and endotoxemia. The aim of this study was to assess oxidative DNA damage and the total antioxidant status (TAS) in peripheral lymphocytes of rats during different intraperitoneal gram-negative sepsis stages. Adult male Sprague-Dawley rats were divided randomly into four groups. Control group was intraperitoneally inoculated with 2 ml, of pyrogene-free saline (Group I, n = 6), and the other rats received an intraperitoneal inoculum with 2 ml, of saline containing 2 x 10(8) CFU of Escherichia coli. The animals were killed at time zero (Group 1, n = 6), at 6th (Group 11, n = 7), 12th (Group M, n = 7), and 24th (Group IV, n = 7) hour after the E. coli inoculation. Oxidative DNA damage in peripheral lymphocytes of rats was evaluated by modified comet assay (single-cell gel electrophoresis). Formamido-pyrimidine DNA glycosylase (Fpg) and Endonuclease III (Endo III) were used to detect oxidized purines and pyrimidines, respectively. Total antioxidant quantification was carried out using ABTS+ (2,2'-Azino-di-[3 ethyl-benzthiazoline sulphonate]) radical formation kinetics (Randox kit) in serum samples. Significant elevations of basal levels of strand breaks (SB) in Group IV were observed as compared with Group I, II, and III. There was a significant increase in Fpg sites in Group III as compared with Group I and II. However, there was no significant difference in terms of Endo III sites in any of the groups. Although the TAS was decreased with the stages of sepsis, this moderate decrease was significant in only Group IV as compared with Group I. There was no statistically significant correlation between DNA damage and TAS for any of the groups.en_US
dc.identifier.citationKaymak, C., Kadioglu, E., Ozcagli, E., Osmanoglu, G., Izdes, S., Agalar, C., Basar, H., & Sardas, S. (2008). Oxidative DNA damage and total antioxidant status in rats during experimental gram-negative sepsis. Human & experimental toxicology, 27(6), 485–491.en_US
dc.identifier.doi10.1177/0960327108088972
dc.identifier.endpage491en_US
dc.identifier.issn0960-3271
dc.identifier.issue6en_US
dc.identifier.pmid18784201
dc.identifier.scopus2-s2.0-55749100848
dc.identifier.scopusqualityQ2
dc.identifier.startpage485en_US
dc.identifier.urihttps://doi.org/10.1177/0960327108088972
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4143
dc.identifier.volume27en_US
dc.identifier.wosWOS:000259373300005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmodified comet assayen_US
dc.subjectoxidative DNA damageen_US
dc.subjectsepsisen_US
dc.subjecttotal antioxidant statusen_US
dc.titleOxidative DNA damage and total antioxidant status in rats during experimental gram-negative sepsisen_US
dc.typeArticle

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